A new green light sensor with high dynamic range
Nic Ong's paper describing the engineering of "CcaSR v3.0", a third-generation version of our E. coli green light sensing system was just published online at ChemBioChem. The Sode group recently showed you can delete two PAS domains of unknown function from the CcaS cyanobacteriochrome light sensor. Here, Nic introduced that deletion into CcaSR v2.0 and it lowered leakiness by 4-fold while increasing dynamic range from 120 to 600-fold! This tool should be useful for a wide range of optogenetic experiments.
Congratulations Dr. Ong!
Nic gave an outstanding thesis defense on "Engineering Bacterial Optogenetic Sensors"! Throughout his PhD, Nic was a tenacious, thoughtful, and innovative researcher. Nic will be missed by all but we can't wait to see what his next endeavors hold for him! Congratulations Dr. Ong!!!
Comprehensive Optogenetic Model Published!
Evan's paper, A photoconversion model for full spectral programming and multiplexing of optogenetic systems, was just published in Molecular Systems Biology. In this paper, Evan built a predictive mathematical model of the spectral responses our E. coli green and red light sensors and used it to program their gene expression output dynamics independently in the same cell. Not only does this work dramatically improve our understanding of how these optogenetic tools function, but the method can be used to perform entirely new characterizations of multi-component gene circuits.
Kat Wins NSF Graduate Research Fellowship!
Tabor lab Senior Kat Sofjan was recently named a National Science Foundation Graduate Research Fellow! The NSF GRFP is a highly prestigious fellowship program that recognizes outstanding scientific contributions and potential in students who will be entering a Ph.D. program the following year. Congratulations, Kat! We are super proud of you.
Rohan Wins Goldwater Fellowship!
Tabor lab Sophomore Rohan Palanki was recently awarded the highly prestigious Barry M. Goldwater Fellowship! The Goldwater is administered by the federal US government to recognize the top science, engineering, and mathematics students in the country each year. This year, Rohan was one of only 240 students selected across the country. This is a great recognition of Rohan's accomplishments and great potential for the future. Congratulations Rohan!!
Kristina's Paper Published
Kristina's paper "Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation" was recently published in Molecular Systems Biology! Along with our former undergraduate Ravi Sheth (currently a Hertz Fellow in Harris Wang's lab at Columbia), Kristina discovered the first genetically-encoded sensor of thiosulfate. She used this sensor to program a gut adapted E. coli strain to sense thiosulfate. Then, with our collaborators the Britton and Shroyer groups at Baylor College of Medicine, she demonstrated that these bacteria sense and report colon inflammation in mice via increased green fluorescent protein expression. This is an exciting early example of the potential of engineered bacteria to study and diagnose gut disesaes, and could eventually be advanced as a therapeutic with high efficacy and low side effects. Congratulations Kristina! Check out the Rice press release.
Kathryn Brink Joins the Lab!
Kathryn Brink, a first year Systems, Synthetic, and Physical Biology PhD student has joined the Tabor Lab. We welcome her with open arms and look foward to the amazing research she'll produce!